Point Mutation CRISPR Planner

Get a complete CRISPR plan for your point mutation request

The Automated CRISPR Planner aims to streamline your CRISPR design process given only the gene name and the amino acid mutation you wish to insert.

The tool is designed for point mutations (one amino acid changed to another) in the Caenorhabditis elegans genome.
The tool has been built to generate CRISPR designs according to the methods described in Geraldine Seydoux's paper:

Paix A, Folkmann A, Goldman DH, et al. Precision genome editing using synthesis-dependent repair of Cas9-induced DNA breaks. Proc Natl Acad Sci U S A. 2017;114(50):E10745-E10754. doi:10.1073/pnas.1711979114

Point Mutation CRISPR Planner

In order to use the service, please insert your worm gene name, nucleotide strand sequence (unspliced + UTR; optional), amino acid site you wish to mutate, the amino acid you wish to alter from and the amino acid you wish to alter to.

If you don't have a valid crRNA sequence with which you wish to plan your CRISPR, please leave that box empty.
Once clicking the "Submit" button, you will be presented with all possible crRNA sequences relevant to your request.
Choose one of the RNA guides presented to you and then you will get the complete CRISPR design plan.

Your worm gene name:

The amino acid site:

The isoform full name (optional): For example: PDR-1 gene has 3 different isoforms: K08E3.7a.1 , K08E3.7b.1 , K08E3.7b.2 , K08E3.7c.1.

The nucleotide strand (unspliced + UTR; optional):

From: To:

Preferred restriction enzymes (no need to add the notation "-HF" for high fidelity): For example: EcoRI, PvuII, BspHI, etc'.

By not providing any relevant restriction enzymes, the tool will search for all known enzymes.

Maximum number of results (per crRNA):

chosen crRNA (if you have already):

Choose strand:

The process can take up to several minutes, please be patient