The Automated CRISPR Planner aims to streamline your CRISPR design process given only the gene name and the amino acid mutation you wish to insert.
The tool is designed for point mutations (one amino acid changed to another) in the
Caenorhabditis elegans genome.
The tool has been built to generate CRISPR designs according to the methods described in Geraldine Seydoux's paper:
Paix A, Folkmann A, Goldman DH, et al. Precision genome editing using synthesis-dependent repair of Cas9-induced DNA breaks. Proc Natl Acad Sci U S A. 2017;114(50):E10745-E10754. doi:10.1073/pnas.1711979114
In order to use the service, please insert your worm gene name, nucleotide strand sequence (unspliced + UTR; optional), amino acid site you wish to mutate, the amino acid you wish to alter from and the amino acid you wish to alter to.
If you don't have a valid crRNA sequence with which you wish to plan your CRISPR, please leave that box empty.
Once clicking the "Submit" button, you will be presented with all possible crRNA sequences relevant to your request.
Choose one of the RNA guides presented to you and then you will get the complete CRISPR design plan.